Michael Hooker Microscopy Facility (MHMF.ORG)
What's Down & Scheduled Repairs
On Going Issues:
Sep 25: Leica SP2 Confocal System - A new computer and hard drive has been installed. Some points worth noting:
Sep 04: Leica SP2 Confocal: System is not useable due to bad x-axis galvo. Engineer is expected Sep 06.
Sep 04: \\Minsky file server - 7:20 to 9:35 PM file server was inaccessible due to an unplugged network cable, which had a missing locking flange. The cable has been replaced.
Apr 19: Leica SP2 confocal - AOBS fans have been replaced.
Mar 27: Olympus FV1000 confocal - the replacement red 638 nm red laser has been installed. All laser in the merge box were alignment. All laser lines are functioning again.
Mar 26: The AD/DS mhmicroscopy (mh-microscopy.med.unc.edu) domain server (Marvin) had a failed mirror drive. The system did not reboot to the remaining mirror until the sATA cables were reversed. The mirror pair has been rebuild.
Mar 19 Laptop wireless access to \\Minsky file shares is not working. This is due to network infrastructure issues beyond our responsibility/control. Access from desktops and laptops plugged into the wired network (Ethernet) works normally.
Mar 05: Olympus FV1000 Confocal Microscope - red laser has failed ( 635 nm for far red, e.g. Alexa 633/647, Draq-5, ToPro-3). Note that the other lasers are unaffected, so dyes such as 405 nm -> DAPI, 440 nm -> CFP, 488 nm -> Alexa488/GFP/FITC, 559 nm -> Rhodamine/TexasRed/Alexa 555/568, etc. can still be imaged.
Feb 19: The web server and calendars were down from 6:20 AM to 9:55 AM. The UPS was found off again. The UPS was replaced.
Feb 05: The web server and calendars were down from 7:05 AM to 8:58 AM. The UPS was found off. We are investigating why it turned off.
Feb 04: Network disruption continue. We need your help in tracking down problems, so let us know if you have issues connecting over the network by telling us which compouter(s) you are trying to connect to, your location and whether you are using a desktop or laptop.
Feb 01: Expect network weirdness. This is due reconfiguring of the VLAN in Thurston Bowles. Problems will roll around different network jacks and hence the associated computers. Once the reconfiguration is finished the weirdness factor should almost go away completely. If you have problems please call so that we can know that there is a problem, and we will try to find a workaround for you.
Jan 31: On Thursday evening several computers could not connect to several other computers in 6th floor of Thurston Bowles building. The disruption lasted about 30 minutes, and should be taken as a warning not to scan images directly to servers over the network. ITS/OIS was notified and a ticket was created.
~ use shift + \
@ use shift + ‘
# use \
\ use ??? we're looking for the key combination
Apr 03 Multiphoton Ti:Sapphire laser is unavailable due to a failed chiller. A replacement is being shipped. We will let you know when there is an expected delivery date is available. The standard lasers are still usable on the Olympus FV1000. Please do not turn on the Mai Tai Ti:Sapph laser.
Mar 22: Multiphoton Ti:Sapphire laser is down due to a flakey chiller unit. Discussion with service is in progress and a replacement is pending. The standard lasers are still usable on the Olympus FV1000. Please do not turn on the Mai Tai Ti:Sapph laser.
Oct 15: Leica SP2 Confocal - A loaner 63X 1.4 NA oil immersion objective is available while the 63x 1.4 NA UV objective is out for repair. Please note the loaner objective may not be well corrective for UV in the z-axis.
As a stop gap while the 63x is out for repair please use 40x and zoom in an extra 1.575x. There will be a slight (11%) loss of x-y resolution which is not subjectively noticeable, and would only be detectable under the most optimum of sample and imaging conditions. Note: If you will be doing x-z scanning with UV please see Michael or Neal about an alternate 40x 1.25 NA objective which has good x-z UV colocalization.
Mar 31: Leica SP2 confocal - Intermittent crashes reported as a SCSI interface error. Service is scheduled for Monday April 5 & now also 6. See note on computer (and below) for the procedure to restart the scanner.
If the machine is not working/giving errors:
Recovery procedure 1:
|Zeiss AIM 510 (full off line)||Installed|
|Leica SP2 (full off line)||Installed|
|QuickTime Pro 7.2||Installed|
|Adobe Creative Suite 3||Installed|
|Roxio DVD/CD writing||Installed|
|Symantec Anti Virus 10.1.6.6010||Installed|
If you experience any problems with the microscope, please document them in the log book and alert a facility director.
Shared Folder Shared Path (Hidden to users) \\minsky\Alessandra F:\Users3\Alessandra \\minsky\Buchman-lab F:\Users3\Buchman-lab \\minsky\CSeagle F:\Users3\CSeagle \\minsky\CWD-lab F:\Users3\CWD-lab \\minsky\Hartnett F:\Users3\Hartnett \\minsky\Leica4D F:\Users3\Viceroy \\minsky\LZhang F:\Users3\LZhang \\minsky\Ostrowski F:\Users3\LEO-lab \\minsky\PGeisen F:\Users3\Hartnett \\minsky\Pickles F:\Users3\Pickles \\minsky\Pickles-lab F:\Users3\Pickles \\minsky\Rubenstein-lab F:\Users3\Rubensteiin-lab \\minsky\Sechelski F:\Users3\Sechelski \\minsky\SKreda F:\Users3\SKreda \\minsky\VanDyke-lab F:\Users3\VanDyke-lab \\minsky\Viceroy F:\Users3\Viceroy
Notes: For administrative purposes this drive is called users3, although users do not have access to this drive though the \\minsky\users3 share.
|\\Minsky\Users3 Users' protected area||F:\Users3|
|200 GByte Firewire 1394 drive|
|\\Minsky\Reid-lab (same as above)||F:\Users3\Reid|
|\\Minsky\Viceroy Leica4D (OLD)||F:\Users3\Viceroy|
Some firewall zones need to be setup. Please contact Michael if you can not connect to \\HOOKE over the network.
The Leica4D confocal (Viceroy) is no longer connected to the Medical School Network, and hence the entire internet, including the Cytic_Fibrosis domain. Scanning can still be performed and the 100 MByte Zip drive can be used to transfer data. OIS has discontinued the sub-network that the 4D was assigned to on Feb. 18, 2003 as a final act in a 5 year plan to give the 152.2. subnet back to the main campus, and block that subnet in the Medical School. Unfortunately the 4D OS9-VME computer can not be easily be migrated to the Medical School subnet. Hence the 4D is isolated from the network. Alternative solutions are being investigated, but are limited by the potential fragility of the two unsupported operating systems that are an essential part of the 4D confocal.
Copyright 2001-2014 Dr. M. Chua, Program in Molecular Biology & Biotechnology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599
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Questions/comments/problems: Michael Chua
Last Updated: 2014-06-24