Probe |
Ex (nm) |
Em (nm) |
|
Reactive and conjugated
probes |
Fluorescein |
495 |
519 |
FITC; pH sensitive |
TRITC |
547 |
572 |
TRITC |
Texas Red |
589 |
615 |
Sulfonyl chloride |
Alexa Fluor dyes
(Molecular Probes) |
Alexa Fluor 350 |
346 |
445 |
|
Alexa Fluor 488 |
494 |
517 |
|
Alexa Fluor 568 |
578 |
603 |
|
Alexa Fluor 594 |
590 |
617 |
|
Alexa Fluor 647 |
650 |
668 |
|
Cy Dyes (AP Biotech) |
Cy2 |
489 |
506 |
|
Cy3 |
(512);550 |
570;(615) |
|
Cy5 |
(625);650 |
670 |
|
Adapted from Salk Flow Cytometry home page
http://flowcyt.salk.edu/fluo.html
|
Probe |
Ex (nm) |
Em (nm) |
|
Nucleic acid probes |
DAPI |
345 |
455 |
AT-selective |
SYTOX Green |
504 |
523 |
DNA |
Propidium Iodide (PI) |
536 |
617 |
|
TOTO-3, TO-PRO-3 |
642 |
661 |
|
Cell function probes |
Fura-2 |
340/380 |
510 |
AM ester. Low/High Ca++, |
Fluo-3 |
506 |
526 |
AM ester. pH > 6, Ca++ |
Fluorescent Proteins |
BCECF |
490 |
535 |
pH |
eBFP |
380 |
440 |
(Clontech) Quantum yield
0.18 |
"GFP" |
396,475 |
508 |
Quantum yield 0.77 |
eCFP |
434 |
477 |
(Clontech) Quantum yield
0.40 |
eGFP |
489 |
508 |
(Clontech) Quantum yield
~0.60 |
eYFP |
514 |
527 |
(Clontech) Quantum yield
0.61 |
Other probes |
mRFP1 |
584 |
607 |
(Tsien
lab) Quantum yield 0.25 |
Calcein |
496 |
517 |
pH > 5 |
|