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Michael Hooker Microscopy Facility (MHMF.ORG)

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Comparison of Confocal Features

Confocal Microscope Laser Lines x-z scanning Image format Viewing software Time lapse Spectral Scanning Frap Fret Location
Leica SP2 aobs upright 351
365
458
477
488
514
568
633
good tiff LCS lite good good ok good Thurston Bowles
(MHMF)
Zeiss 510 Meta inverted 458
477
488
514
543
633
ok zeiss tiff Image Browser
(Zeiss)
good good good ok Thurston Bowles
(MHMF)
Perkin Elmer Yokogawa spinning disk inverted 488
568
647
no tiff   +       Thurston Bowles
(MHMF)
Leica 4D inverted 351
365
488
568
633
good tiff           Thurston Bowles
(MHMF)
LeicaTCS-NT inverted & upright 477
488
568
633
good tiff LCS lite +       MBRB
Zeiss 510 NLO upright 488
543
IR
2 photon
no zeiss Image Browser
(Zeiss)
+       Neurosci Research Building

All systems can do serial sectioning by x-y scanning and changing focus.

Viewing software is available for use on PCs running Windows and is free.


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Copyright 2001-2014  Dr. M. Chua, Program in Molecular Biology & Biotechnology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599
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Last Updated: 2006-01-24