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Making Measurements with ImageJ

Outline: 

This measurement protocol provides an example of how to measure the percent of black areas in a field of cells stained with phalloidin.  Images need not be calibrated since a percentage is being estimated.  Black areas are estimated using a intensity threshold. Another possible way to estimate black areas is to manually outline areas by hand.  However this method is not presented here (yet?).

Summary:

Protocol:


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Copyright 2001-2014  Dr. M. Chua, Program in Molecular Biology & Biotechnology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599
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